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From Victoria, It's actually a relatively straight-forward route to the southern highlands. Hop on the Hume Freeway and that will take you all the way to just 5 minutes from the region! From there, jump on the A48 Illawarra Highway, which will take you all the way into town. From Sydney, it's the same deal but beginning at the other end of the Hume! Nice and simple.
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From Victoria, there is a V-line train & Bus service that takes you to Goulburn, where you can connect to the Southern Highlands rail line. From Sydney, take the T8 line (that also goes via the airport) to Glenfield, where there is a Bus service that will take you directly to Moss Vale!
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To keep it simple, you can fly into either Sydney or Canberra, both of which sit 1.5 hours away from the area. From there, you can either drive or catch a bus / train the remainder of the way. Otherwise, there is a flight from Essendon Fields airport direct to Wollongong which is just 20 minutes from the Southern Highlands area!